Effect of Palmitic Acid on Tertiary Structure of Glycated Human Serum Albumin

Non-enzymatic glycation is a process, which can be best described as significant posttranslational modification of various proteins. It emerges in hyperglycemic conditions and may have an impact on albumin stability well its activity physical chemical properties, essentially affecting all physiological functions. The goal this research was to answer the following questions: (i) how does defatted human serum by glucose–fructose syrup (GFS) alter tertiary structure; (ii) palmitic acid (PA), component palm oil, affect vitro process cause conformational changes glycated albumin; (iii) PA inhibit formation Advanced Glycation End-Products (AGEs)? Therefore, order point out differences structure macromolecules, absorption emission fluorescence spectra their second derivatives, excitation synchronous spectra, Red-Edge Excitation Shift (REES effect), degree sulfhydryl groups defatted, non-glycated (HSA) (gHSA) with GFS were investigated. In present study, it has been confirmed that presence causes both HSA gHSA structures, respectively. Moreover, acid, at ratios 1.5:1 3:1 albumin, not exhibit inhibition AGEs formation. This study indicates fact structural changes, especially those are important for treatment planning because type interaction between components primary transporter altered disease progresses or elderly.